Modification of Platelet Shape Change Parameter by Modified Phospholipids in Oxidized LDL
Werasak Sutipornpalangkul PhD, MD*, Supeenun Unchen PhD**, Yupin Sanvarinda PhD**, Udom Chantharaksri PhD**
Affiliation : * Department of Orthopaedic Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand ** Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
Background : It is believed that the oxidatively modified lipoproteins play a critical role in activating platelets
and is a contributing factor in the etiology of a number of cardiovascular-related diseases.
Objective : Identify the active component(s) of oxidized LDL that initiated shape change in plasma-free human
platelets prepared by a gel filtration method.
Material and Method: Shape change parameter of platelets was monitored following exposure platelets to
LDL, copper sulfate-oxidized LDL, and different types of lipids extracted of the corresponding LDL.
Results : Oxidized LDL, but not native LDL, increased the shape-change parameter in a concentration-depen-
dent manner. Specifically, phosphatidyl serine from oxidized LDL was responsible for this effect.
Conclusion : Oxidized phospholipids generated during the oxidative modification of LDL are likely to be the
active components responsible for changes in platelet function.
Keywords : Oxidized LDL, Platelet shape change, Phospholipids, Atherosclerosis
