N-Acetylcysteine Inhibits Proliferation, Adhesion, Migration and Invasion of Human Bladder Cancer Cells
Athikom Supabphol MBBS*, Verasing Muangman MD**, Warinthorn Chavasiri PhD***, Roongtawan Supabphol PhD****, Wandee Gritsanapan PhD*****
Affiliation : * Interdisciplinary Programme in Clinical Sciences, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand ** Department of Surgery, Vichaiyut Hospital and Medical Center, Bangkok, Thailand *** Department of Chemistry, Faculty of Sciences, Chulalongkorn University, Bangkok, Thailand **** Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand ***** Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
Objective : Bladder cancer is not only a major public health and economically burden for the patients but
also a major clinical impact for Thai urologists. The authors’ aim was to study the anti-metastatic effect of
N-acetylcysteine (NAC), one of the cheap, safe and widely used over-the-counter-drugs in Thailand, on the
human bladder cancer cells.
Material and Method: Effects of NAC at various concentrations on the growth, adhesion, migration, and
invasion of the human bladder cancer cell line were assessed in vitro.
Results : NAC at the concentrations of 5, 10, 20 and 30 mM could directly and significantly inhibit the growth,
adhesion, migration, and invasion of the human bladder cancer cells in a dose-dependent manner. The 50%
inhibitory concentration (IC50) value for cell viability was 33.33 + 0.78 mM. The inhibitory effects on migration,
invasion and adhesion properties of the cancer cells were dramatically observed at the concentrations of
> 10, > 20, and > 30 mM respectively.
Conclusion : NAC has an anti-metastatic effect on the human bladder cancer cells by inhibiting their growth,
adhesion, migration, and invasion properties. This implies the high possibility that the urologists may apply
the results to use it intravesically before, during and after the transurethral resection of bladder tumour, in
addition to its conventional usage by oral and parenteral routes.
Keywords : N-acetylcysteine, Cell adhesion, Cell migration, Cell proliferation, Cell invasion, Urinary bladder neoplasms
