Effect of Curcumin on Vascular Endothelial Growth Factor Expression in Diabetic Mice Kidney Induced by Streptozotocin
Tarinee Sawatpanich MSc*, Hattaya Petpiboolthai MSc*, Busaba Punyarachun PhD*, Vipavee Anupunpisit PhD*
Affiliation : * Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand
Objective : To localize and demonstrate the effect of curcumin on vascular endothelial growth factor in diabetic
mice kidney induced by streptozotocin
Material and Method: Diabetic mice were induced by streptozotocin (60 mg/kg BW). Male mice were divided
into three groups, control mice, diabetic mice (DM) and diabetic mice treated with curcumin (DMC) (200 mg/
kg BW). At 4 and 8 weeks, animals were sacrificed and kidneys were processed by immunohistochemistry
technique.
Results : At the end of 4 and 8 week experiments, glomeruli were slightly enlarged and showed diffuse thicken-
ing of the glomerular capillary walls in diabetic mice. Administration with curcumin presented the better
improvement and recovery of cells and tissues compared with diabetic mice. Immunohistochemical staining
for vascular endothelial growth factor (VEGF) demonstrated that VEGF was mainly detected in the podocytes
and renal tubules. There was an increase in VEGF expression in diabetic mice as compared to control.
Treatment with curcumin significantly inhibited the expression of VEGF in the kidney tissue of diabetic mice
in both 4 and 8 weeks. Comparing the diabetic mice between 4 and 8 week experiments, the expression of
VEGF in the podocytes and renal tubules at 8 week were significantly stronger than at 4 week which repre-
sented time-dependent change. Nevertheless, the intensity of VEGF was not different in DMC mice when it was
compared between 4 and 8 weeks.
Conclusion : VEGF immunoreactivity of the podocytes and the renal tubules at 4 and 8 weeks in DM mice
showed strong intensity more than in control mice. However, the intensity of VEGF in DMC mice was less when
it was compared with DM mice. Moreover, VEGF was a key modulator of angiogenesis and a potent mitogen
for endothelial cells. These results demonstrated the potential use of antiangiogenic curcumin as a novel
therapeutic agent in diabetic mellitus and maintain normal structure of the kidney.
Keywords : Curcumin, Vascular endothelial growth factor, Streptozotocin, Immunohistochemistry
