Home-Made Rapid Urease Test Compared with Commercial Kit for Diagnosis of Helicobacter pylori Infection
Chatchawan Wongjitrat MD*, Nattakarn Wongjitrat MD**
Affiliation : * Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand ** Division of Pediatric Endocrinology, Department of Pediatric, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand
Objective : To evaluate the sensitivity and specificity of home-made rapid urease test compared with commercial kit for diag-
nosis of Helicobacter pylori infection.
Material and Method: A cross-sectional study of patients who underwent esophagogastro- duodenoscopy from June 2009 to
May 2010 was carried out. Gastric biopsy specimens were taken from antrum and body of the stomach for home-made rapid
urease testing, commercial kit (hpfast) urease testing, and histological study.
Results : One hundred and ninety nine patients were included in the present study. The sensitivity of home-made rapid urease
test and hpfast were 40% and 48.5% at 1 hour and 91.4% and 91.4% at 24 hours. The specificity, positive and negative
predictive value and accuracy of home-made rapid urease test vs. hpfast were 100% vs. 100%, 100% vs. 100%, 95.5% vs.
95.5% and 96.9% vs. 96.9% respectively. Furthermore 45.3% of home-made rapid urease test and 54.7% of hpfast showed
positive results within 1 hour.
Conclusion : Home-made rapid urease test has good sensitivity and specificity comparable to commercial kit for the
diagnosis of H. pylori infection.
Keywords : Helicobacter pylori, Home-made rapid urease test, Hpfast, Diagnostic test
