Anti-Allergic, Anti-Inflammatory and Antioxidant Activities of the Different Extracts of Thai Traditional Remedy Called Prabchompoothaweep for Allergic Rhinitis Treatment
Areeratna Jai-aue BATM*, Sunita Makchuchit MSc*, Thana Juckmeta MSc*, Arunporn Itharat PhD**,***
Affiliation : * Graduate School, Faculty of Medicine, Thammasat University, Pathumthani, Thailand ** Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, Thailand *** Center of Excellence on Applied Thai Traditional Medicine Research (CEATMR), Faculty of Medicine, Thammasat University, Pathumthani, Thailand
Background : Prabchompoothaweep remedy (PT) has long been used in Thai traditional medicine to treat allergic rhinitis
and asthma. It is composed of 23 plants. It is on National herbal drug list of Thailand, but there is no report for anti-allergic,
anti-inflammatory and antioxidant activities.
Objective : To investigate anti-allergic, anti-inflammatory and antioxidant activities of the crude extract from PT by different
extraction method.
Material and Method: The method of extract used was maceration in 95% ethanol and 50% ethanol; the residue of these
extracts were continued extracted by boiling water, they obtained PTE95, PTE50, PTR95 and PTR50, respectively. The other
method of extraction was boiling and drying by lyophilizer that obtained PTW. Five crude extracts were determined anti-
allergic activity by the inhibition of β-hexosaminidase release from RBL-2H3 cell lines, anti-inflammatory activity were
determined by the inhibition of nitric oxide (NO) production from RAW 264.7 cell lines induced by lipopolysaccharide (LPS)
and antioxidant activity were tested by DPPH radical scavenging assay.
Results : PTE95 showed the most potent of anti-allergic activity, anti-inflammatory activity and antioxidant activity (IC50 =
12.97, 22.51 and EC50 = 14.62 μg/ml, respectively).
Conclusion : These results suggest that the method of extraction PT that showed the best anti-allergy, anti-inflamation and
antioxidant activity was maceration in 95% ethanol.
Keywords : Anti-allergic activity, β-hexosaminidase, Anti-inflammatory activity, Nitric oxide, Antioxidant activity, Prabchompoothaweep remedy, Extract
