Acute and Sub-Chronic Toxicity of Tri-Sa-Maw Recipe in Rats
Urarat Nanna MS*, Kanjana Jaijoy PhD**, Nirush Lertprasertsuke PhD***, Noppamas Soonthornchareonnon PhD****, Seewaboon Sireeratawong PhD*,*****
Affiliation : * Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Rungsit Campus, Pathumthani, Thailand ** McCormick Faculty of Nursing, Payap University, Chiang Mai, Thailand *** Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand **** Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand ***** Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
Background : Tri-sa-maw recipe is a botanical preparation comprised of equal proportions of the three herbal fruits, namely
Terminalia chebula Retz., Terminalia sp. and Terminalia bellirica Roxb. This recipe is used for antipyretic, expectorant,
periodic maintenance, and relieving stomach tight.
Objective : To evaluate the acute and sub-chronic toxicities of Tri-sa-maw recipe extract in rats.
Material and Method: In the present study of acute toxicity, a single oral dose 5,000 mg/kg of Tri-sa-maw recipe extract was
administered to rats. Sub-chronic toxicity was studied by the daily oral administration of the extract at the doses of 600, 1,200
and 2,400 mg/kg body weight for consecutive 90 days.
Results : Tri-sa-maw recipe extract at the dose of 5,000 mg/kg showed no signs of differences as compared to the control rat.
No abnormalities were found in the sub-chronic toxicity study; none of the parameters for body and organ weights, hematol-
ogy, blood chemistry, necropsy, and histopathology showed any differences between the control and all treatment groups.
Conclusion : Tri-sa-maw recipe extract did not significantly cause acute toxicity or sub-chronic toxicity in rats.
Keywords : Tri-sa-maw recipe, Acute toxicity, Sub-chronic toxicity
