An Investigation of Antimicrobial and Wound Healing Potential of Allium ascalonicum Linn.
Suphaket Saenthaweesuk MSc*, Rungrat Jitvaropas PhD*, Nuntiya Somparn PhD*, Amornnat Thuppia MSc*
Affiliation : * Department of Preclinical Science, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, Thailand
Background : The pharmacological properties of Allium ascalonicum Linn., commonly called shallot, have been reported as
including those that are antibacterial and antioxidant.
Objective : The present study aimed to evaluate the antimicrobial effect and wound-healing activity of the ethanolic extracts of
Allium ascalonicum Linn. (AAE).
Material and Method: The antimicrobial activity of AAE was tested in vitro against using the disc diffusion method and a
broth micro-dilution technique to determine the minimal inhibition concentrations (MIC) and the minimal microbicidal
concentrations (MMC). Wound-healing activity of the extract was performed on rat test subjects.
Results : The AAE showed potential antimicrobial activity by inhibiting gram-positive bacteria Staphylococcus epidermidis
and Bacillus subtilis ATCC 6633. MIC and MMC varied from 25-50 mg/ml and 25-200 mg/ml, respectively. After surgery
14 days, wound contractions of treated groups and standard group were 78.61+1.20%, 78.55+1.93% and 100%, respectively;
but, in the control group, wound contraction was 64.90+3.55%. Histological studies showed the complete epidermis and
found the collagen fibers and fibroblasts as similar appearance as standard group in dermis. The results of histological
evaluation have confirmed remarkable wound-healing activities of AAE.
Conclusion : Taken together, the present study provides evidence that AAE extract processes antimicrobial and wound-
healing activities.
Keywords : Allium ascalonicum Linn., Antimicrobial activity, Wound-healing
