Urinary Leukotriene E4 Level in Non-Allergic Thai Young Children
Phinidda Cha-umphol MSc*, Paskorn Sritipsukho MD**,***, Sirikul Manochantr PhD****, Siripat Kiatpunsodsai MD*****
Affiliation : * Doctor of Philosophy Degree student in Medical Sciences Program, Faculty of Medicine, Thammasat University, Pathumthani, Thailand ** Department of Pediatrics, Faculty of Medicine, Thammasat University, Pathumthani, Thailand *** Center of Excellence in Applied Epidemiology, Thammasat University, Pathumthani, Thailand **** Divisions of Cell Biology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, Thailand ***** Department of Surgery, Faculty of Medicine, Thammasat University, Pathumthani, Thailand
Background : Urinary leukotriene E4 (uLTE4) concentration represents body leukotriene synthesis. The level increases
especially in respiratory allergic diseases. Researches of leukotriene production role in children with respiratory allergic
diseases required the normal levels in non-allergic children as references.
Objective : The study was to assess the reference values of uLTE4 in non-allergic Thai children measured by competitive
enzyme-linked immunosorbent assay (ELISA) technique.
Material and Method: Children who were admitted for elective surgery, aged 6 months to 5 years, were included in the study.
Subjects who had acute illness, respiratory allergy (allergic rhinitis and asthma), and chronic diseases were excluded.
Morning urine (5 ml) was collected for uLTE4 analysis by competitive ELISA technique.
Results : There were 36 urinary samples from 36 subjects aged 6-60 months with 26 boys and 10 girls. The mean of uLTE4
concentration was 619.73+701.32 pg/ml and the mean of uLTE4 concentration adjusted for urinary creatinine concentrations
was 1,328+788.54 pg/mg creatinine. The mean of uLTE4 concentration in boys and girls were 1,349+817.10 pg/mg
creatinine and 1,275+747.79 pg/mg creatinine respectively. The mean of uLTE4 concentration adjusted for urinary creatinine
concentrations were 1,363+886.65, 1,384+771.81 and 1,223+773.16 pg/mg creatinine for children with the age of 6-18, 19-
36 and 37-60 months respectively.
Conclusion : The concentrations of uLTE4 and uLTE4 per creatinine were presented by age group in Thai very young
children with non-respiratory disease. The concentrations were elevated in younger age group.
Keywords : Urine leukotriene E4, Creatinine, Reference, Children, Non-allergic
