Induction of mESCs into Hepatic Stem Cells by using Embryonic Chicken Hearts
Sanong Suksaweang MT, MS, PhD*, Danna Ye MS**, Rangsun Parnpai PhD**
Affiliation : * Department of Pathology and Laboratory Medicine, Institute of Medicine; Suranaree University of Technology, Nakhon Ratchasima, Thailand ** Department of Biotechnology, Institute of Agricultural Technology; Suranaree University of Technology, Nakhon Ratchasima, Thailand
Background : Many researchers have been trying different methods for obtaining stem cells. Some studies have failed due to
the growth of a tumor after stem cells transplantation. Several successful tries for getting stem cells or stem cell like cells:
direct isolation from tissue, direct isolation from blood or fluids, iPS cells, small molecules induced stem cells. However, none
have used real organ stimulation in the induction of a specific stem cell lineage.
Objective : To induce a lineage specific hepatic stem cell using isolated embryonic organs.
Material and Method: The embryonic stem cells were cultured through confluence. After observing several colonies formations,
we put freshly isolated chicken embryonic hearts onto the colonies. After, at least, four days, we started looking for hepatic
plate-like formations.
Results : After several trials, we found that the chicken embryonic hearts, on day 4, could actually induce a hepatic cell fate for
the mouse embryonic stem cells. We were able to show specific marker for early hepatic lineage such as the production of
Albumin, AFP. When these cells were tested for a hepatocyte function, we found glycogen formation inside the cells.
Conclusion : Isolated early embryonic chicken hearts are acceptable for inducing embryonic stem cells into the hepatic stem
cell lineage.
Keywords : Mouse embryonic stem cells, Hepatic stem cells, Early embryonic chicken heart
