Screening of Potential Probiotic Lactic Acid Bacteria with Anticancer Properties
Juntana Chimchang MSc*, Talent Theparee MD*, Sinutchanan Wongein MSc**, Techin Trivirot MD*, Somboon Tanasupawa PhD***, Malai Taweechotipatr PhD*, Benjamas Wongsatayanon PhD*
Affiliation : * Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand ** HRH Princess Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand *** Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Background : Probiotics have shown to reduce cancer recurrence and side effects in colorectal cancer patients.
Objective : To isolate the lactic acid bacteria from Thai healthy newborn feces and screen good probiotics with anticancer
properties.
Material and Method: Lactic acid bacteria were isolated from newborn feces and selected for the cytotoxicity property
against human cancer cell lines by MTT assay and probiotics property by acid and bile tolerance tests.
Results : Among 200 lactic acid bacteria isolated, 3 and 1 isolates significantly demonstrated strong and moderate anti-
proliferative effect against Caco-2 cells, respectively. Likewise, 4 and 5 isolates showed significant strong and moderate
inhibitory effect on U937 cells, respectively. Seven candidate strains did not displayed cytotoxic to normal cells (Vero), except
MSMC 112-2. All candidates showed good probiotics properties in resistance to acidic condition (pH 2-4) and to 1-4% bile
concentrations, except MSMC105-3 showed intolerance at 4% bile concentration. The nucleotide sequence homology
showed that MSMC95-4, MSMC104-2, MSMC111-2, MSMC112-2 and MSMC215-1 strains belong to Enterococcus faecalis
(99.4%, 98.8%, 99.5%, 98.7 and 98.9%, respectively), MSMC105-3 is Lactobacillus salivarius (99.1%) and MSMC171-1
is Enterococcus faecium (99.3%).
Conclusion : The authors have isolated lactic acid bacteria which have anticancer and good probiotics properties.
Keywords : Lactic acid bacteria, Probiotics, Anticancer, Caco-2 cells, U937 cells
