Anti-Proliferation and Apoptosis Induction in Breast Cancer Cells by Cratoxylum cochinchinense Extract
Sukanda Innajak BSc*, Sirinun Nilwarangoon PhD*, Wilawan Mahabusarakam PhD**, Ramida Watanapokasin PhD*
Affiliation : * Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand ** Department of Chemistry, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
Background : Breast cancer is the most common invasive cancer in females worldwide. It was found about 37.5% in Thai
females and is one of the leading causes of death-related cancers in women. Therefore, new finding of anti-cancer compound
as a therapeutic candidate in breast cancer is necessary.
Objective : To investigate the effect of Cratoxylum cochinchinense extract on anti-proliferation and apoptosis induction in
breast cancer cells.
Material and Method: Cell proliferation and cell viability assay were determined by MTT assay. Hoechst 33342 and JC-1
staining were used to determined nuclear morphological changes and mitochondrial membrane potential, respectively.
Results : C. cochinchinense extract showed anti-proliferation in MDA-MB-468 treated cells in a time- and dose-dependent
manner with IC50 value of 19.19+0.8 μg/ml. In addition, C. cochinchinense extract induced nuclear condensation and
apoptotic bodies in MDA-MB-468 treated cells. JC-1 staining revealed that C. cochinchinense extract induced mitochondrial
membrane dysfunction.
Conclusion : C. cochinchinense extract showed anti-proliferation and apoptosis induction properties in MDA-MB-468
treated cells. These results suggested that C. cochinchinense extract may be a potential candidate for anti-cancer drug
developing. The underlying mechanisms of apoptosis induction should be further studied.
Keywords : Breast cancer cells, C. cochinchinense extract, Apoptosis, Anti-proliferation
