Autologous Fibrin-Base Scaffold for Chondrocytes and Bone Marrow Mesenchymal Stem Cells Implantation: The Development and Comparison to Conventional Fibrin Glue
Channarong Kasemkijwattana MD*, Visit Rungsinaporn MD*, Thana Siripisitsak MD*, Pinkawas Kongmalai MD*, Ronnachit Boonprasert MD*, Chatchavan Charoenthamruksa MD*, Suradej Hongeng MD**, Adisak Wongkajornsilp MD***, Soranart Muangsomboon MD****, Kanda Chaipinyo PhD*****, Kosum Chansiri PhD******
Affiliation : * Department of Orthopaedics, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand ** Department of Pediatrics, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand *** Department of Pharmacology, Siriraj Hospital, Mahidol University, Bangkok, Thailand **** Department of Pathology, Siriraj Hospital, Mahidol University, Bangkok, Thailand ***** Faculty of Health Science, Srinakharinwirot University, Nakhon Nayok, Thailand ****** Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand
Objective : The authors developed the autologous fibrin-base scaffold for chondrocytes and bone marrow mesenchymal stem
cells (BM-MSCs) implantation and evaluated cells viability in autologous fibrin-base scaffold comparing to commercial fibrin
glue.
Material and Method: The chondrocytes and BM-MSCs were seeded into autologous fibrin-base scaffold and commercial
fibrin glue. The cell viability and proliferation were evaluated at 1 and 7 days. The histology were evaluated with hematoxylin-
eosin (H&E) staining and cartilaginous matrices formation with Alcian blue, Saffanin-0, Toluidine blue, and Collagen type II
staining at 6 weeks. The fixation of the scaffolds was observed.
Results : The chondrocytes and BM-MSCs could not survive in commercial fibrin glue. The chondrocytes and BM-MSCs in
autologous fibrin-base scaffold could proliferate and synthesize the cartilaginous matrices on Alcian blue, Saffanin-0,
Toluidine blue, and Collagen type II staining at 6 weeks. The fixation strength is excellent.
Conclusion : The developed autologous fibrin-base scaffold can be used as the scaffold for chondrocytes and BM-MSCs
implantation with potential to implant chondrocytes and BM-MSCs arthroscopically.
Keywords : Knee injury, Autologous chondrocytes implantation, Bone marrow mesenchymal stem cells (BM-MSCs), Fibrin- base scaffold
