Chromosome Analysis of Uncultured Amniocytes by Comparative Genomic Hybridization in Early Amniocentesis
Aphornpirom Ketupanya MD*, Niwat Aranyakasemsuk MD*, Chintana Tocharoentanaphol PhD**, Chutawadi Vuthiwong MD*
Affiliation : * Departments of Obstetrics and Gynecology, Phramongkutklao Hospital, Bangkok 10400, Thailand ** Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Objective : To study chromosome analysis by comparative genomic hybridization (CGH) compared with
the conventional technique in early amniocentesis.
Materials and Methods : Cross-sectional descriptive study design was performed in 32 singleton pregnant
women with gestational age between 12-15 weeks. Transabdominal amniocentesis was carried out under
ultrasound guidance. The amniotic fluid samples were simultaneously investigated using CGH and the
conventional cytogenetics study as a gold standard.
Results : Amniocentesis were done for advanced maternal age in all cases. The mean maternal age was
35.8 years (35-42 years). The mean gestational age was 13.7 weeks (12-15 weeks) .The chromosome
analysis by CGH technique of uncultured amniocyte showed 17 normal female chromosomes (53.1%)
and 15 normal male chromosomes (46.9%). This finding was the same as the conventional cytogenetics
method. The mean duration of the CGH method was 6 days and that of the conventional cytogenetics
method was 13.7 days (10-23 days).
Conclusion : The CGH technique is a reliable technique for a rapid prenatal diagnosis of chromosome
study in early gestation.
Keywords : Chromosome, Early Amniocentesis, Comparative Genomic Hybridization
