Proteomic Analysis of Oletf Rats, Type 2 Diabetic Animal Model
Jung Hwa Jung1, Jong Ryeal Hahm1, Mi Yeon Kang1, Sung Won Moon1, Deok Ryong Kim2,
Affiliation : 1Department of Internal Medicine, Gyeongsang National University Hospital, Jinju, South Korea, 2Department of Biochemistry, College of Medicine, Gyeongsang National University, Jinju, South Korea
Background and Aims. The OLETF (Otsuka Long-Evans Tokushima Fatty) rat is a useful animal model of type
2 diabetes with obesity. This rat has a feature induced diabetes after 18 weeks.
Materials and Methods. To understand proteins related with diabetes, total proteins, from liver, muscle, fat
and pancreas of 6-week (normal) and 18-week (diabetic) OLETF rats, were analyzed by two-dimensional gel
electrophoresis.
Results. According to the comparative analysis of proteins differentially expressed in normal and diabetic
OLETF rat’s tissues, diabetic pancreas have 200 proteins 3-fold more over expressed and 120 proteins 3-fold
more repressed than normal. Among these proteins, while expression of Rho GDP dissociation inhibitor
(GDI)-·, phosphatidylethanolamine-binding protein and peroxiredoxin-2 increased, alpha-amylase, caldecrin
precursor, nuclear hormone precursor, carboxypeptidase B, bile salt activated lipase precursor, protein disul-
fide isomerase precursor and chymotrypsin B was decreased. Furthermore, liver, muscle and fat tissue showed
the increased protein expression at 89, 72 and 170 spots, respectively, at the diabetic condition and decrease
of many proteins in diabetes.
Conclusion. Such findings might provide some information to understand the molecular mechanism and
develop new drugs for diabetes.
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