Lipid Peroxidation and Renal Injury in Renal Ischemic Reperfusion: Effect of Angiotensin Inhibition
Yuyen Seujang MSc*, Wipawee Kittikowit MD**, Somchai Eiam-Ong MD***, Somchit Eiam-Ong PhD*
Affiliation : * Department of Physiology, Faculty of Medicine, Chulalongkorn University **Department of Pathology, Faculty of Medicine, Chulalongkorn University ***Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University
Objectives : To investigate the role of angiotensin inhibition on lipid peroxidation (LPO) and renal pathology
in ischemic reperfusion (IR).
Materials and Methods : Male Wistar rats were subjected to 15-, 30-, 45- or 60- minutes of renal ischemia (I) by
left renal artery occlusion. In the 30-minute I group, reperfusion (R) for 1 day (I30R1) was performed in
additional animals that had been treated with water, angiotensin converting enzyme inhibitor (ACEI; enalapril
5 mg/kg/d), or angiotensin receptor type 1 blocker (ARB; losartan 10 mg/kg/d) one day before I and were
continued for 1 day after R. Renal tissue malondialdehyde (MDA), an indicator of LPO, was examined during
I and IR periods. Renal pathology was also determined.
Results : During ischemia, renal tissue MDA levels were increased throughout the 60-minute ischemic period
and was maximum at 30 minutes of ischemia (p < 0.01). Histological changes in 30-minutes I group showed
slight tubular cell congestion and mild interstitial edema. One day after reperfusion, MDA levels were still
elevated (p < 0.01) when compared with sham. Progression of renal pathology was observed after 1 day of
reperfusion. Both ACEI and ARB could attenuate the heightened MDA levels (p < 0.01). IR-induced renal
injury was markedly diminished by administration of ACEI as well as by ARB.
Conclusion : These results indicate that inhibition of angiotensin could reduce lipid peroxidation and amelio-
rate renal injury during IR condition.
Keywords : Lipid peroxidation, Renal injury, Renal ischemic reperfusion, Angiotensin inhibition
