Vitrification of Mouse Oocyte Using Open Pulled Straws Compared with Needles
Surachai Pornwiroon MD*, Somboon Kunathikom MD*, Orawan Makemaharn MSc*, Rattanaphorn Huanaraj BSc*
Affiliation : * Department of Obstetrics and Gynecology, Faculty of Medicine, Siriraj Hospital, Mahidol University
Objective : To compare the survival rate of mouse oocytes and fertilization rate between using open pulled
straws (OPS) and needles for vitrification.
Materials and Methods : Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and
allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphologi-
cal survival and fertilization rate were examined.
Results : There was no obvious difference between the morphological survival rates of vitrified mouse oocytes
using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95%CI -7.1, 10.7). The vitrified
oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%).
Proportions Difference -14.3% (95%CI -24.5, -3.6).
Conclusion : Vitrification method of mouse oocytes using needles when compared to OPS provides a similar
morphological survival rate and higher fertilization rate.
Keywords : Mouse oocytes, Oocyte vitrification, Open pulled straws, Needles, Survival rate, Fertilization rate.
