Analysis of Connective Tissue Growth Factor Promoter Polymorphism in Thai Children with Biliary Atresia
Pattaratida Sa-nguanmoo MSc*, Paisarn Vejchapipat MD, PhD**, Voranush Chongsrisawat MD*, Chintana Chirathaworn PhD***, Sittisak Honsawek MD, PhD ****, Apiradee Theamboonlers BSc*, Yong Poovorawan MD*
Affiliation : * Center of Excellence in Viral Hepatitis Research, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University ** Pediatric Surgery Unit, Department of Surgery, Faculty of Medicine, Chulalongkorn University *** Department of Microbiology, Faculty of Medicine, Chulalongkorn University **** Department of Biochemistry, Faculty of Medicine, Chulalongkorn University
Objective : Connective tissue growth factor (CTGF) has been proposed to play a key role in the pathogenesis
of hepatic fibrosis in biliary atresia (BA). The aim of the present study was to determine the single nucleotide
polymorphism (SNP) in the promoter region of CTGF gene in a Thai population, and to investigate the
possible role of CTGF promoter polymorphism in the susceptibility of BA.
Materials and Methods : Genomic DNA was obtained from 84 patients with BA and 142 healthy controls. The
-447G/C and -132C/G in CTGF promoter were amplified and examined by amplification-refractory mutation
system (ARMs) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analy-
sis, respectively. The test of Hardy-Weinberg equilibrium (HWE) was performed using HWE program of
SNPAnalyzer. Statistical analysis was carried out with SPSS and Epi Info.
Result: According to the previous experiment, there were two SNPs, which were at position -447 and -132 on
the promoter. However, there was only one SNP at the position -447 in the Thai population. No significant
differences in genotype and allele frequency were observed between BA and controls or with BA subgroups.
Conclusion : The present study demonstrated that CTGF polymorphism at -447G/C was not associated with
BA and the jaundice status of the postoperative BA patients.
Keywords : Biliary atresia, CTGF, SNP, Polymorphism
