Effects of N-Acetylcysteine on Oxidative Stress in Rats with Non-alcoholic Steatohepatitis
Suchittra Samuhasaneeto MSc*, Duangporn Thong-Ngam MD*, Onanong Kulaputana MD, PhD*, Suthiluk Patumraj PhD*, Naruemon Klaikeaw MD**
Affiliation : * Department of Physiology, Faculty of Medicine, Chulalongkorn University ** Department of Pathology, Faculty of Medicine, Chulalongkorn University
Objective : Prove the attenuated effects of N-acetylcysteine (NAC) on oxidative stress in rats with nonalco-
holic steatohepatitis (NASH).
Materials and Methods : Male Sprague-Dawley rats were randomly divided into five groups. Group 1 (normal
control) was fed regular dry rat chow (RC) for 6 weeks. Group 2 (NASH) was fed 100% fat diet for 6 weeks.
Group 3-5 were fed 100% fat diet for 6 weeks, and then switched to RC alone (NASH+diet ; group 3), to
RC+20 mg/kg/day of NAC orally (NASH+diet+NAC20 ; group 4) or to RC+500 mg/kg/day of NAC orally
(NASH+diet+NAC500 ; group 5) for 4 weeks, respectively. They were sacrificed to collect blood and liver
samples at the end of the present study.
Results : Levels of total glutathione (GSH), serum cholesterol, and hepatic malondialdehyde (MDA) were
increased significantly in the NASH group compared with normal control. Liver histopathology from group 2
showed moderate to severe macrovesicular steatosis, hepatocyte ballooning, and necroinflammation. Treat-
ment with diet or diet plus NAC reduced the levels of GSH, cholesterol, and hepatic MDA back to normal. Liver
sections from group 3-5 showed a decrease in fat deposition and necroinflammation in hepatocytes. However,
no differences on all variables existed between diet alone and diet plus NAC groups.
Conclusion : Our data indicate that diet or diet plus NAC treatment could attenuate oxidative stress and
improve liver histopathology of NASH. However, the addition of NAC is not better than diet treatment alone.
Keywords : NASH, Steatohepatitis, N-acetylcysteine, Oxidative stress
