The Study of Human Skin Irritation of a Novel Herbal Skin Care Product and Ingredients by Human Single closed Patch Testing
Penpun Wattanakrai MD*, Suthida Suwanachote MD*, Sutinee Kulkollakarn MD*, Natta Rajatanavin MD*
Affiliation : * Division of Dermatology, Department of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok
Background : The Government Pharmaceutical Organization of Thailand (GPO) has developed many products
using liposome nanotechnology and Thai herbal extracts.
Objective : Evaluate the irritation potential of GPO products on human skin using the single application
closed patch test under occlusion. The authors also studied the moisturizing efficacy of a commercial curmin
extract cream (GPO curmin cream).
Materials and Methods : Thirty-six female volunteers were tested with 12 test materials developed by GPO
including liposome, curmin extract: tetrahydrocurcuminoids (THC), and commercial curmin cream. Two and
a half percent sodium dodecyl sulfate (SDS) was used as positive control. Standard Finn chambers on Scanpor
tape with webril cotton were used as occlusive patch test devices. Cutaneous irritation responses were graded
after patch removal and the incidence of irritation compared to the positive control was used for evaluation.
Corneometer was used to measure skin hydration before and after application of curmin cream.
Results : All volunteers completed the present study. The skin irritation effects from the test materials were
significantly lower (p-value < 0.001, McNemar statistic test) than the positive control. Measurement of skin
hydration after twice daily application of GPO curmin cream was significantly higher (p-value < 0.001,
paired t-test) than the control skin.
Conclusion : The test materials and finished products developed by the GPO are not likely to induce skin
irritation under normal conditions of use. Furthermore, twice-daily application of the commercial GPO
curmin cream can significantly increase skin hydration.
Keywords : Herbal extracts, Curmin, Tetrahydrocurcuminoids, Irritation, Hydration
